The Natarajan laboratory investigates the fundamental process of cell fate specification during development and differentiation at single-cell level. We focus on understanding general paradigms of cell state and fate specification, with a particular interest in crosstalk between cell cycle and transcriptional regulation.
We apply and develop a wide variety of experimental and computational tools to study mechanisms involved in cell fate specification in embryonic stem cells, induced pluripotent cells but also across cellular differentiation. These bulk and single-cell approaches include advanced molecular biology, flow cytometry, genome editing and variety of single-cell genomics and multi-omics methods. Our research aims to gain a deeper ‘system biology’ single-cell view of biological systems.
We study how transcriptional regulation is governed globally during cell cycle and across phases in ESCs/iPSCs at single-cell level.
We utilise cell cycle sensors combined with candidate gene perturbation and single-cell profiling to understand different mechanisms involved in cell cycle regulation of pluripotency and differentiation.
Distinct cell cycle changes accompany stem cell differentiation and can drive cell-fate responses.
We combine state-of-art single-cell genomics and computational tools to investigate transcriptional and epigenetic changes that accompany cell-fate specification.
We have a strong interest in development, extension and utilisation of new experimental and computational methods for single-cell biology.
Working closely with collaborators, we develop new computational tools that integrate measurements from several single-cell technologies.
MSc: Valentina Hekimova, Maja Brask, Sophia Hald
BSc: Maja Brask, Felix Pedersen
PhD: Simon Jakobsen, Andreas Moller
Postdoc: Deyong Zhu
Our research is possible due to generous support form:
We are part of DTU Bioengineering
at Technical University of Denmark.
Transcriptional regulation and Single-cell systems genomics,
Technical University of Denmark
Søltofts Plads, DK-2800 Kgs. Lyngby