Natarajan Laboratory
Exploring the single-cell interplay between cell cycle, transcriptional regulation and cell fate specification.
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Our research

The Natarajan laboratory investigates the fundamental process of cell fate specification during development and differentiation at single-cell level. We focus on understanding general paradigms of cell state and fate specification, with a particular interest in crosstalk between cell cycle and transcriptional regulation.

We apply and develop a wide variety of experimental and computational tools to study mechanisms involved in cell fate specification in embryonic stem cells, induced pluripotent cells but also across cellular differentiation. These bulk and single-cell approaches include advanced molecular biology, flow cytometry, genome editing and variety of single-cell genomics and multi-omics methods. Our research aims to gain a deeper ‘system biology’ single-cell view of biological systems.

"Simply, we study how individual cells during development and differentiation divide, scale in numbers to generate an incredible complexity and types of cells, tissues and organs."

Areas of research

Cell cycle control of transcriptional regulation

We study how transcriptional regulation is governed globally during cell cycle and across phases in ESCs/iPSCs at single-cell level.
We utilise cell cycle sensors combined with candidate gene perturbation and single-cell profiling to understand different mechanisms involved in cell cycle regulation of pluripotency and differentiation.

Cell fate specification and dynamics during differentiation

Distinct cell cycle changes accompany stem cell differentiation and can drive cell-fate responses.
We combine state-of-art single-cell genomics and computational tools to investigate transcriptional and epigenetic changes that accompany cell-fate specification.

New methods for single-cell biology

We have a strong interest in development, extension and utilisation of new experimental and computational methods for single-cell biology.
Working closely with collaborators, we develop new computational tools that integrate measurements from several single-cell technologies.

Our Team

Kedar Natarajan

Group Leader

HyunTae Choi

Guest researcher

Da Eun Lee

Guest researcher


MSc: Valentina Hekimova, Maja Brask, Sophia Hald

BSc: Maja Brask, Felix Pedersen

PhD: Simon Jakobsen, Andreas Moller

Postdoc: Deyong Zhu

Join us!

We welcome enquiries from highly interdisciplinary, motivated and ambitious candidates.

Postdocs candidates with strong background and interest in areas of single-cell genomics, computational biology and machine learning are welcome to enquire informally on lab projects. We encourage and support applications to Danish and international funding agencies. Please email a tailored cover letter, CV, publication record and details of two references

Interested PhD students are also welcome to enquire about applying for funding. Please email directly.



TENET: gene network reconstruction using transfer entropy reveals key regulatory factors from single cell transcriptomic data.

Kim J, Jakobsen ST, Natarajan KN# and Won KJ#
Published in: Nucleic Acid Research.


Predicting gene regulatory networks from cell atlases

Moeller AF and Natarajan KN#
Published in: Life Science Alliance.

SBML Level 3: an extensible format for the exchange and reuse of biological models

Keating SM, Waltemath D, et al., and SBML Level 3 Community members
Published in: Molecular Systems Biology.

Transcriptional dynamics of hepatic sinusoid‐associated cells after liver injury.

Terkelsen MK, Bendixen SM, Hansen D, Scott EAH, Moeller AF, Nielsen R, Mandrup S, Schlosser A, Andersen TL, Sorensen GL, Krag A, Natarajan KN, Detlefsen S, Dimke H, Ravnskjaer K
Published in: Hepatology.

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression.

Cuomo SE, Seaton DD, McCarthy DJ, Martinez I, Bonder MJ, Garcia-Bernardo J, Amatya S, Madrigal P, Isaacson A, Buettner F, Knights A, Natarajan KN, HipSci Consortium, Vallier L, Marioni JC, Chhatriwala M, Stegle O.
Published in: Nature Communications.

Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development.

Oatley M, Bolukbasi OV, Svensson V, Shvartsman M, Ganter K, Zirngibl K, Pavlovich PV, Milchevskaya V, Foteva V, Natarajan KN, Baying B, Benes V, Patil KR, Teichmann SA, Lancrin C
Published in: Nature Communications.


The Malaria Cell Atlas: Single parasite transcriptomes across the complete Plasmodium life cycle.

Howick VM, Russell AJC, Andrews T, Heaton H, Reid AJ, Natarajan K, Butungi H, Metcalf T, Verzier LH, Rayner JC, Berriman M, Herren JK, Billker O, Hemberg M, Talman AM, Lawniczak MKN.
Published in: Science.

Comparative analysis of sequencing technologies for single-cell transcriptomics.

Natarajan KN*#, Miao Z*, Jiang M*, Huang X, Zhou H, Xie J, Wang C, Qin S, Zhao Z, Wu L, Yang N, Li B, Hou Y, Liu S, Teichmann SA#.
Published in: Genome Biology.

Single-Cell Tagged Reverse Transcription (STRT-Seq).

Natarajan KN#.
Published in: Methods in Molecular Biology.


A rapid and robust method for single cell chromatin accessibility profiling.

Chen X, Miragaia RJ, Natarajan KN, Teichmann SA.
Published in: Nature Communications.

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Pramanik J, Chen X, Kar G, Henriksson J, Gomes T, Park JE, Natarajan K, Meyer KB, Miao Z, McKenzie ANJ, Mahata B, Teichmann SA.
Published in: Genome Medicine.

Distinctive features of lincRNA gene expression suggest widespread RNA-independent functions.

Tuck AC, Natarajan KN, Rice GM, Borawski J, Mohn F, Rankova A, Flemr M, Wenger A, Nutiu R, Teichmann SA, Bühler M
Published in: Life Science Alliance.


Single cell transcriptomics of pluripotent stem cells: reprogramming and differentiation.

Natarajan KN*#, Teichmann SA, Kolodziejczyk AA.
Published in: Current Opinion of Genetics and Development.

Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression.

Kar G, Kim JK, Kolodziejczyk AA, Natarajan KN, Torlai Triglia E, Mifsud B, Elderkin S, Marioni JC, Pombo A, Teichmann SA
Published in: Nature Communications.

SC3: consensus clustering of single-cell RNA-seq data

Kiselev VY, Kirschner K, Schaub MT, Andrews T, Yiu A, Chandra T, Natarajan KN, Reik W, Barahona M, Green AR, Hemberg M.
Published in: Nature Methods.

Cell biology: tracking a cell’s cycle

Natarajan KN*# and Teichmann SA (Journalistic feature)
Published in: Nature methods.

Postdocs: What would you tell your younger self.

Natarajan KN*# (Special feature comment)
Published in: Cell.

Power analysis of single-cell RNA-sequencing experiments.

Svensson V*, Natarajan KN*, Ly LH, Miragaia RJ, Labalette C, Macaulay IC, Cvejic A, Teichmann SA.
Published in: Nature Methods.


Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation.

Proserpio V, Piccolo A, Haim-Vilmovsky L, Kar G, Lönnberg T, Svensson V, Pramanik J, Natarajan KN, Zhai W, Zhang X, Donati G, Kayikci M, Kotar J, McKenzie AN, Montandon R, Billker O, Woodhouse S, Cicuta P, Nicodemi M, Teichmann SA.
Published in: Genome Biology.

The systems biology format converter.

Rodriguez N, Pettit JB, Dalle Pezze P, Li L, Henry A, van Iersel MP, Jalowicki G, Kutmon M, Natarajan KN, Tolnay D, Stefan MI, Evelo CT, Le Novère N.
Published in: BMC Biology.


Single Cell RNA-Sequencing of Pluripotent States Unlocks Modular Transcriptional Variation.

Kolodziejczyk AA, Kim JK, Tsang JC, Ilicic T, Henriksson J, Natarajan KN, Tuck AC, Gao X, Bühler M, Liu P, Marioni JC, Teichmann SA
Published in: Cell Stem Cell.

Computational assignment of cell-cycle stage from single-cell transcriptome data.

Scialdone A, Natarajan KN, Saraiva LR, Proserpio V, Teichmann SA, Stegle O, Marioni JC, Buettner F
Published in: Methods.

Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells.

Buettner F*, Natarajan KN*, Casale FP, Proserpio V, Scialdone A, Theis FJ, Teichmann SA, Marioni JC, Stegle O.
Published in: Nature Biotechnology.


BioModels: ten-year anniversary.

Chelliah V, Juty N, Ajmera I, Ali R, Dumousseau M, Glont M, Hucka M, Jalowicki G, Keating S, Knight-Schrijver V, Lloret-Villas A, Natarajan KN, Pettit JB, Rodriguez N, Schubert M, Wimalaratne SM, Zhao Y, Hermjakob H, Le Novère N, Laibe C
Published in: Nucleic Acid Research.


The System Biology Format Converter.

Jalowicki G, Rodriguez N, Kutmon M, Pettit JB, Li L, Henry A, Natarajan KN, Laibe C, Evelo CT, Novere NL
Published in: Nature Precedings.


Our research is possible due to generous support form:

Contact Us

We are part of DTU Bioengineering at Technical University of Denmark.

Transcriptional regulation and Single-cell systems genomics,
DTU Bioengineering
Technical University of Denmark
Søltofts Plads, DK-2800 Kgs. Lyngby